We propose that these enzymes present a modular structure in their active sites that can be dissected into two halves: one providing the attacking nucleophile, and the second one stabilizing a negatively charged reaction intermediate.
Brains were either divided sagittally into two equal halves, or dissected into individual regions [olfactory bulb, frontal cortex (anterior to Bregma, dorsal grey matter containing little or no white matter), hippocampus, cerebellum and brain stem] along with cervical spinal cord.
Dissected into 20 sections.
The small intestine was dissected into duodenum, jejenum and ileum.
A microscalpel and iridectomy scissors were used to dissect cochlear halves into apical, middle, and basal half-turns.
The stellate ganglion was dissected in two halves, thus separating the first thoracic part from the inferior cervical part.
Briefly, calvariae were dissected from 2-day-old mouse pups, cut into halves, and separated from dura mater, vessels, and scalp.
Each fruit was sectioned into halves under a dissecting microscope.
Strips (1 cm) of third intrapulmonary generation neonatal pulmonary arteries from PPHN and control piglets (9 12 strips per animal) were gently dissected under sterile conditions, denuded of endothelium and cut longitudinally into halves.
Calvarial bones were dissected from 6- to 7-day-old mice (CsA), divided into halves along the sagittal suture, and preincubated with α-MEM with 0.1% albumin, antibiotics, and 1 μmol/l indomethacin overnight.
