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After Bentham's death, in accordance with his directions, his body was dissected in the presence of his friends.
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Renton agrees that "there is no electronic substitute for students talking together and exchanging thoughts and ideas in order to dissect them in the presence of a senior leader".
Ten 5 to 7 day-old females were housed with 30 wild-type CS males in a food vial for 3 h for mating and then the female reproductive system was dissected to examine the presence of an egg in the oviduct or uterus.
The cerebellum was dissected in the midline of the vermis.
Flies were then dissected, the abdomen cut and opened in the presence of 20 mM N-ethylmaleimide.
For isolation of PND0 prostates, urogenital tracts were removed and VPs microdissected in a modified watch glass (Maximov depression slide; Fisher, Pittsburgh, PA, USA) in the presence of dissecting media [basal medium of Dulbecco's modified Eagle's medium and Hams F-12 (1:1 vol/vol) supplemented with penicillin and streptomycin (5 mL/L) and fungizome (20 μg/mL) at pH 7.3].
Retinas from newborn mouse, embryonic day 4 chicken, and 82 day, and 90 96 day fetal human retinas (obtained from the Laboratory of Developmental Biology - NIH HD 000836 at the University of Washington without identifiers) were dissected from extra-ocular tissues and cultured in the presence of IRBP-GFP lentivirus as free-floating explants in ultra-low attachment plates overnight.
Embryonic tissue was dissected, digested with papain, and dissociated by trituration in the presence of DNAse I. Papain was quenched with ovomucoid albumin inhibitor and cells were collected via filtration and centrifugation.
Finally, we used the latest RefSeq mRNA annotation dataset for whole human transcriptome (build of July 18 , 2011 for the purpose of evaluating Dissect's false event discovery rate in the presence of short-to-medium size novel insertions.
Therefore, the subsequent experiments were designed to dissect the regulatory mechanisms underlying hepcidin expression pattern in the presence of an active UPR.
Postnatal tissue was dissected, digested with 0.25% trypsin, dissociated by trituration in the presence of DNAse I, and cells were collected via filtration and centrifugation.
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