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For in situ hybridization, brains were immediately removed from terminally anesthetized animals [overdose of ketamine (50 mg/ml), xylazine (10 mg/ml), and atropine (0.1 mg/ml)] and the whole visual cortices were quickly dissected (area determined according to [ 29, 30]), embedded in Tissue Tek (Sakura Finetek, Heppenheim, Germany), flash frozen in liquid nitrogen, and stored at -80°C.
The dissected area of the chromosomes was found to be finer and sharper compared with the conventional AFM probe.
One year later, we found that axons from the proximal spinal cord regenerated, traversed the dissected area inside the tube and reentered the distal spinal cord, leading to functional restoration of the essentially paralyzed hind limbs.
We chose midbrain-brainstem samples (Figure 4A) since the dissected area includes the affected nuclei.
The dissected area was estimated to contain the corresponding potential injured area and was restricted to avoid dilution of the sample with RNA from non-relevant brain regions.
Within the dissected area, GFP-expressing cells were present only in the region between hilus and granule cells layer or SGZ (Fig. 1c).
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In southern São Paulo the Serra do Mar is characterized by three distinct terrain types: 1) highly dissected areas with closely spaced ridges and accordant summit heights; 2) multiconvex hills; and 3) terrains with highly elevated watershed areas, irregular summit heights, and locally subdued relief.
Our cutoff level in cancer sections was achieving ⩾80% tumour cells in the dissected areas.
Dissected areas were collected in the cap of a microcentrifuge tube via laser pressure catapulting.
An isolated neck imaging-detected relapse developed in previously dissected areas in six patients and in areas that had not been dissected in the other two.
In our previous and present studies, the extended field of irradiation was used to cover the three field dissected areas by extended surgery in Japan.
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