Dissected material was stored for as many as several days at −80°.
Reverse transcription of RNA from manually dissected material was performed using 500 ng of RNA for each reaction.
For dissected material, seed coat (20 mg), endosperm (20 mg), embryo: 15 DAP (10 mg), 20 DAP (50 mg) and older (100 mg) was used.
Expression of TLR4, IL1B, IL6, IL8, and SAA in manually dissected material and LCM material was highly correlated (Pearson's r > 0.72, P < 0.05); CD14 and TNF expression was also positively correlated in the two sample types, although not significantly.
One mcrolitre cDNA from the liver and T cells or 2-4 μL of cDNA from laser dissected materials were used in each of the following RT-PCR reactions as templates.
And she is able to dissect this material lucidly until she leaps into overdrive, declaring the existence of a concerted pro-Gore plot.
The exceptionally large size of maize anthers makes it straightforward to dissect sufficient material for biochemistry.
Alongside in class discussions, students will participate in dissecting cadaveric material with the aim to build hands-on musculoskeletal knowledge.
Porcine genes that displayed high expression in the high-throughput gene expression analysis of manually dissected lung material were selected for analysis in LCM samples.
For cuticle removal, dissected flower material was moved through a Histo-Clear (National Diagnostics, Atlanta, Georgia, USA) series (v/v, Histo-Clear/ethanol) (25%, 50%, 75 %, 100) for 1 h at each stage.
Stardust plans to drop off the sample when it swings by Earth in January 2006, and cosmochemists will then take on the daunting challenge of dissecting nanoscale materials that hold clues to the solar system's formation and possibly to life's starting materials.
