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This so-called microRNA disrupts the expression of two key cellular genes called TGF-β and SMAD3, in a process known as RNA interference (RNAi).
Interpretation of our results may therefore be limited by some possibility that the SENP3-MO also disrupts the expression of other mRNAs.
Thus, the Dutch patients are still able to express the isoform 2 from this mutant allele, while the other allele (carrying the IVS7+1G/T splice mutation) disrupts the expression of both isoforms.
RT-PCR was performed to confirm that formaldehyde inhalation exposure significantly disrupts the expression of miRNAs.
Each stock contains one P element that disrupts the expression of a gene and causes recessive lethality.
The sbj3 allele introduces a premature stop codon and disrupts the expression of the full-length protein.
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Both regimens of E2 disrupted the expression of spatial memory in place-learners following the novel start position.
Gene fusions in cancer typically lead to the expression of a fusion protein or disrupt the expression of one of the parental genes.
Our data indicated that in Japanese rice fish, ethanol disrupted the expression of only cnr1a in a concentration-dependent manner that induced delay in hatching and might be responsible for the development of FASD phenotypes.
Dale's team is also looking at how gene insertion itself may alter transgenic plants, for example by disrupting the expression of endogenous plant genes close to the location of the insertion.
After selection with puromycin (0.5 µg/mL) for 2 days, FACS was performed to analyze the expression levels of the dual fluorescence markers, which showed that NLS-TALENs were highly efficient in targeting nuclear sequences and disrupted the expression of EGFP in 13%20%% of the transfected cells.
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