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Targeted disruption was determined by Southern blot and long-range PCR analysis (Fig. 7 B C).
A relative measure of BBB disruption was determined by calculating the percentage of pixels with a contrast enhancement above 20%% within the brain on calculated percent difference images.
The specificity for ER- α disruption was determined by transfecting the three siRNAs into Hep3B and HCCLM3 cell lines using FUGENE HD according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA).
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The mean effect of random and hub gene disruptions was determined by measuring the growth rate of each disruption strain within these two groups (figure 1).
Cell disruption capability was determined to be highly dependent on pulse pressure with no significant effects being seen from the influence of number of passes and biomass concentration.
For yeast disruption process, it was determined that the process was independent from effects of biomass concentration at a range below 750 g/L [ 19].
The board statement quoted Douglas Kubach, chief executive of Pearson Educational Measurement, as saying that the company regretted "the uncertainty and disruption these issues caused" and was "determined to take every possible necessary step to restore confidence in this process".
To examine whether the maintenance of telomerase holoprotein integrity by PKC is crucial for enzyme function, the enzyme activity was determined after disruption of the hTERT-hsp90 association by novobiocin and its reassociation by PKC activator SC-10.
Residual gp140T-his remaining at the end of the time-course was determined by disruption of the ICMV particles with 1% Triton X-100 treatment and measuring released protein by fluorescence spectrophotometry.
Membrane binding of α-Synuclein was determined by mechanical disruption of the cells followed by 196,000× g ultracentrifugation of the postnuclear supernatant.
The function of the CoxG gene product was determined by gene disruption in Oligotropha carboxidovorans as anchoring the CO dehydrogenase to the cytoplasmic membrane [ 27].
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