Hearts were disrupted by mechanical tissue disruption using a tissue grinder (Polytron).
People have taken to social media to warn fellow travellers of disruption, using the hashtag #weatherbomb.
If so, he and Mr Friis will have to find another industry that is ripe for disruption using peer-to-peer technology.
Biomass harvesting using filtration and recovery of metabolites without cell disruption using solvent extraction from wet biomass was observed as the best downstream processing strategy.
After cell disruption using Mini-Beadbeater-16 (Biospec, Bartlesville, PA, USA), the suspension was centrifuged, and the supernatant was applied to NucleoSpin® RNA II Kit (Macherey-Nagel, Düren, Germany).
Cell extracts were obtained by disruption using sonication (two cycles, 7 min each) with a TOMY UD-200 sonicator and centrifugation at 13700 x g for 10 min at 4°C.
The modified Pitcher method included the lysis of actinomycetes cells obtained with the pretreatment with lipase (2 mg/ml) followed by the cell wall disruption using lysozyme (200 mg/ml final concentration) and proteinase K (300 μg/ml final concentration) in the presence of 3% sodium dodecyl sulfate (SDS).
We investigated whether the metacyclic trypomastigote stage-specific surface molecule gp82, a Ca2+-signal-inducing molecule implicated in host cell invasion, displayed the ability to induce actin cytoskeleton disruption, using a recombinant protein (J18) containing the full-length gp82 sequence fused to GST.
Thus, as expected, vesicular motion was arrested by microtubule disruption using nocodazole.
The cells were collected when evident cytopathic effect (CPE) was observed followed by disruption using four freeze and thaw cycles.
In this study, we proved that targeted gene disruption using ZFN technology works well and provides for several advantages and possibilities when used in rats.
