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A possible explanation for this lack of significance is the variation in the mean response of the different disruption types to different environmental stresses revealed by the significant interaction between environment and disruption type.
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Six empirical damage categories were identified, ranging from no apparent surface damage (Type 1) to penetration accompanied by complete lay-up disruption (Type 6).
Fig. 6b shows that for thin tephra falls there is a higher probability of tolerance (IS0) and disruption type impacts (IS1).
1 Disruption type was treated as a fixed effect.
To examine mutational robustness, genotype was nested within disruption type and secondary mutation was nested under strain and disruption type.
To examine environmental robustness, disruption type and environment were tested individually and as an interaction term, and strain (i.e. disrupted gene) was nested under disruption type.
There was no consistent effect of disruption type on growth rate in a reference environment.
Therefore, although there was no overall effect of disruption type on mean response to stress, this response did vary over environments in a way that depended on disruption type.
An effect of disruption type (hub or random) on variation in growth rate can be tested by analyzing the significance of the variation among strains response to environmental stress nested within disruption type.
Variance components of the strain effect were 0.014 and 4.9 × 10-5 in the hub and random disruption type analyses, respectively.
Disruption type was treated as a fixed factor, genotype, secondary mutation and environment were treated as random factors.
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CEO of Professional Science Editing for Scientists @ prosciediting.com