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During this period, study follow-ups were on-going to minimize disruption to study flow.
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Our general aim is to establish a reliable barrier disruption method to study the long-term effects on barrier recovery after topical application of pharmaceuticals.
Unfortunately, few genes of turbot and other flatfish have been sequenced, which limits the use micro-arrays and qRT-PCR to study disruption of endocrine responses.
The results suggest that HEPM cells are suitable to study disruption of cell-cell communication as a mechanism responsible for teratogenesis and offer the unique possibility to apply human embryonal cells.
Unfortunately, there are relatively few genes of turbot and other flatfish in GenBank, which limits the use of molecular tools such as microarrays and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) to study disruption of endocrine responses in sentinel fish captured by regulatory agencies.
The findings suggest that PCB3 and PCB5 have significant feminization effects on gonadal differentiation in X. laevis and that this species is sensitive to endocrine disruption and may be used as a good model to study endocrine disruption.
They had been inserting genetic fragments at random in fly DNA to study disruptions in a different gene.
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Since the loss-of-function effect of injected MOs usually lasts for 2 to 4 days [12], such systems can be efficiently used to study how disruption of RP gene translation affects the early embryogenesis of vertebrates.
The generation of mouse knockouts has been a commonly used approach to study gene disruption in the context of a whole organism.
It was also used in ex vivo corneas using a forward detection configuration to study thermal disruption of the collagen lamellae [ 14].
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