Sentence examples for disruption protocol from inspiring English sources

Total RNA was isolated from 5 × 107 cells using the RNeasy MINI kit (mechanical disruption protocol; QIAGEN, Hilden, Germany).

After collection, all samples are processed with the QIAGEN RNeasy Kit to extract the RNA (mechanical disruption protocol with on-column DNase digestion).

The extraction of total RNA was performed according to QIAGEN's Mechanical Disruption Protocol for the isolation of total RNA from yeast using the RNeasy Midi Kit.

Further, the associated gene disruption protocol should not only serve as a guide for chromosomal gene inactivation studies involving mobile group II introns, but also prove invaluable for applying metabolic engineering strategies to C. pasteurianum.

To test the predicted involvement of the indicated genes, a mutant (Figure S6) was generated using previously developed D. radiodurans disruption protocols [75].

Consequently, bespoke nutritional markers used in gene disruption protocols and reporter genes had to be developed for use in this organism (Table 3).

Total RNA was extracted from heads using RNeasy mini (QIAGEN; animal tissue protocol; disruption by squashing heads with a disposable pestle in buffer RLT).

To effectively evaluate a chemical's potential for endocrine disruption, a testing protocol must be able to measure hormone-like effects or perturbations at very low chemical doses, as well as to take into account the many possible ways the chemical may interact with endocrine signaling pathways.

America's space agency, NASA, has been researching what it calls the delay- (or disruption-) tolerant network protocol, or DTN.

Our results show that optimization of expression, disruption, and purification protocols resulted in a higher yield of functional MtbSD enzyme.

The "smash and grab" rapid yeast genomic DNA isolation protocol utilizes disruption of the yeast cell wall by enzymatic digestion or physical fractionation of cells with small glass beads [ 6].