Sentence examples for disruption procedure from inspiring English sources

A one-step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain (UBI3/Δubi3::hisG), previously isolated in the first step of the ura-blaster protocol.

This disruption procedure was followed by DNA extraction with a DNeasy Plant Mini kit (Qiagen) following the manufacturer's recommendations.

In the E.coli.S30 system, a chemical disruption procedure (EDTA) is introduced to dissociate the ribosome and release the mRNA, which is then purified before RT-PCR.

This membrane fraction characteristically showed a bilamellar morphology (i.e. with two interacting membrane bilayers; Fig. 2D) indicating to a stronger membrane interaction which remained intact after the cell disruption procedure and subsequent differential centrifugation steps during their isolation.

However, due to the washing procedure preliminary to the cell harvesting and disruption procedure, this effect could not be observed in the chosen experimental set-up.

Our cluster disruption procedure affects sessile hemocytes indiscriminately such that we cannot determine the relative importance of these cluster features on crystal cell differentiation.

The disruption procedures were shown in Additional file 9.

Ideally, the cell disruption procedures should be standardised by automation, to maintain reproducibility throughout the homogenisation procedures.

However, the enzyme was rapidly degraded after cell lysis in Tris/HCl buffer which is commonly used in cell disruption procedures, although a protease inhibitor was already added.

Some techniques, such as sonication and rupturing with glass beads, employ relatively harsh disruption procedures whose action may dislodge membrane-associated proteins, and thus yield positive activity in the soluble fraction.

Direct visualization of the airway via flexible bronchoscopy did not reveal worsening tracheal disruption throughout the procedure.