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To differentiate between latently infected cells and infectious virus in the samples, serial threefold dilutions of spleen cells were plated before or after mechanical disruption of viable cells (by two freeze-thaw cycles).
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Tissue disruption and recovery of viable protoplasts was in K3 medium.
Mice with homozygous disruption of p53 (p53−/−) are viable except for an exencephalic phenotype in females (Armstrong et al, 1995; Clarke et al, 1993; Donehower et al, 1992).
Unlike the targeted disruptions of Smad2 and 4 which are lethal, mice with targeted disruption of Smad3 are viable and survive to adulthood (up to approximately 8 months of age).
Some of the results are marginally relevant to any aspect of aging, e.g. experiment E-GEOD-6570 E-GEOD-6570 E-GEOD-6570ice whichtareportedisrupthat of ZAS3 are viable with life span comparable to controls'mice
We report that mice with targeted disruption of exon 3 (ErgΔEx3/ΔEx3) are viable, fertile and do not display any overt phenotype.
Although an initial disruption of ndhF is a viable hypothesis to explain ndh loss in both Pinus and Welwitschia, the gene disrupting inversions could not be held in common between the gnetophyte and Pinaceae plastomes because, as discussed earlier, none of the inversions can be shared.
Decreased disruption of the epidermal barrier and areas of viable tissue around each microthermal treatment zone enable more rapid healing and reduce the risk of infection.
P582 is a marked P-element disruption of Hsp90 [32], which is not viable when homozygous and likely makes no Hsp90 protein [33].
In an age of disruption, the only viable strategy is to adapt and the ability to make shifts in platforms and ecosystems is becoming a core business skill.
Glynne and colleagues [ 73] incubated proximal tubular epithelial cells with TNF-α and observed disruption of the actin cytoskeleton and elongation of cells and shedding of viable, apoptotic and necrotic cells dependent on nitric oxide (NO).
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