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Now the researchers plan to investigate whether diseases such as bronchitis or cystic fibrosis are caused by the disruption of the two proteins' partitioning.
The resultant recombinant bacteria were screened for disruption of the two different target loci by growing on LB plates supplemented with kanamycin and chloramphenicol.
Our attempts to generate GLOI null mutants by step wise gene replacement failed despite the successful disruption of the two alleles by gene targeting.
In P. berghei, simultaneous disruption of the two SERA genes does not affect parasite growth [39]; however, substantial expression of these two genes, as observed in this study, cannot rule out their importance in rodent parasites.
Sec61α is part of a trimeric complex, and having confirmed that disruption of the gene by both P-element and RNAi leads to elevated autophagy, we next sought to test the effects of disruption of the two other subunits, Sec61β and Sec61γ.
An important observation in this study was disruption of the two circuits by advancing the IVC cannula into the SVC.
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Sage, J. et al. Targeted disruption of the three Rb-related genes leads to loss of G(1) control and immortalization.
We used a null mutant line of P. patens abi3 which contains no detectable ABI3 due to the disruption of the three copies of the gene [ 46].
The effect of disruption of the six GALT gene family members on adherent seed mucilage was investigated by staining hydrated seeds with ruthenium red, which stains negatively charged biopolymers such as pectin [ 39].
In this work, we analyzed the modifications of the photosynthetic apparatus in the Arabidopsis mutant chy1chy2lut2lut5, that accumulates β-xanthophylls despite disruption of the three chy1, chy2 and lut5 genes encoding carotene hydroxylases.
The RNase R gene transcript level was increased in the Argonaute disruptant, but the disruption of these two genes produced different mRNA abundance patterns according to the tree clustering analysis.
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