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Mechanical disruption of samples was performed using the TissueLyser equipment (Qiagen).
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For all tissue samples, RNA was extracted with an optimized protocol for liver samples using mechanical disruption of tissue samples with zirconium beads and subsequent silica-based RNA extraction, as described elsewhere [ 10].
Scanning electron micrographs illustrated the severely structural disruptions of sample cells by PSE.
The cells were sonicated (three bursts of 20 s each) for further disruption of the samples.
Furthermore, SEM and FTIR results illustrated the severe structural disruption of seed samples by MILT-MHD.
No mechanical disruption of the samples took place during the treatment.
This result in particular seeded the interest in an additional in-depth study to investigate evidence of mechanophysical disruption of these samples at multiple scales.
In these sagittal sections, implanted cPEG maintained distinct linear surfaces, whereas ongoing cAAPEG degradation led to significant cellular infiltration, surface roughness, and disruption of original sample geometry.
As the concentration of phosphoric acid was increased, an increase in the degree of disruption of the sample, including the splitting, delaminating and roughening of the fibres was apparent.
The free oscillation amplitude is set to a low value (~ 5 nm); this is important for a minimal energy input during imaging, thus preventing disruption of the sample and allowing stable imaging.
Briefly, frozen tissues were homogenized in 1 ml TRI Reagent (Ambion; Grand Island, NY, USA) using a hand-held rotor-stator homogenizer to provide efficient disruption and homogenization of samples.
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