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After 4 weeks of streptozotocin and Cholesterol rich diet administration, vascular endothelium dysfunction was assessed, in terms of attenuation of acetylcholine-induced, endothelium-dependent relaxation (Isolated Aortic Ring Preparation), a decrease in serum nitrate/nitrite level, as well as mRNA expression of eNOS (rtPCR) and disruption of integrity of vascular endothelium (Electron microscopy).
After four weeks of l-methionine administration, vascular endothelium dysfunction was assessed in terms of attenuation of acetylcholine-induced endothelium dependent relaxation (Isolated aortic ring preparation), a decrease in serum nitrite level, mRNA expression of eNOS (rtPCR) and disruption of integrity of vascular endothelium (Electron microscopy).
If a larger spot/line separation is set, the laser may induce corneal relaxation rather than corneal dissection by the disruption of integrity of the cornea due to the consequent unconnected microcavitations in the stroma.
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Usually, metal ions inhibit the activity of important enzymes or disruption of the integrity of the cell membrane.
Therefore, exposure of the cells to dGNPs leads to the disruption of the integrity of the cell membrane, causing increased permeability and possible leaching of the cell materials.
It was stated that large cationic PAMAM dendrimers induce platelet aggregation through the disruption of membrane integrity.
Disruption of the integrity of this protein complex may lead to alterations of INaL in pathological conditions.
The L-counterpart of D-MPI mainly exerts its antitumor, antibacterial and antifungal activity through the disruption of the integrity of cell membrane (Wang et al. 2008, 2009, 2013, 2014).
The fluorescence assay performed using PI also showed the disruption of the integrity of the cell membrane which causes increased permeability and possible leaching of the cell materials (Figure7).
In addition, the loss of membrane permeability, release of 260 nm absorbing material, and leakage of K+ ions to the outside can be sensitive indicators of the disruption of membrane integrity (Cox et al. [2001]).
Morphological characteristics, including the disruption of membrane integrity, decreased cell growth, cytoplasmic condensation, and cell clumping, were observed in MCF-7 cells treated with AgNPs, whereas control cells remained active.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com