Sentence examples for disruption method from inspiring English sources

We cloned a putative A. cellulolyticus creA gene that is highly similar to the creA genes derived from other filamentous fungi, and isolated a creA disruptant strain by using the disruption method.

In order to confirm whether the cloned gene cluster is really involved in the biosynthesis of bafilomycin, the gene for a dehydratase domain of module 12 (DH12) of PKS and an FkbH-like gene in S. griseus chromosome was disrupted by PCR-targeted gene disruption method (Gust et al. 2003).

Although like many bacteria (Hughes et al. 1971; Chisti and Moo-Young 1986; Benov and Al-Ibraheem 2002; Donsì et al. 2009), P. putida cells are routinely disrupted in the laboratory using French-press, no detailed analysis of this disruption method has been published.

LPS was isolated using an aqueous membrane disruption method.

Here, a putative MspI endonuclease gene, ccel2866, was inactivated by a ClosTron-based gene disruption method.

Ultra Turrax was the best cell disruption method, and refrigeration was the best preservation method.

In more harsh disruption methods such as grinding and bead beating, the PBs were also disrupted and consequently could not be recovered by density separation.

Several disruption methods were investigated to maximize total RNA extracted from viable cells.

Various simple and fast cell disruption methods were evaluated for the efficient disruption of lactobacilli and pediococci.

For example, while Cas9 can be used to "knock out" genes through error-prone sequence disruption, methods to precisely insert or replace sequences are still in their infancy.

The objective of this study was to compare the effects of several cell disruption methods on the activity of PepX.