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We categorized each mutation as either nonsense, missense, synonymous, splice signal disruption, frame shifting indel, frame preserving indel, or noncoding mutation.
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FASTY comparison to known OR peptide sequences was used to generate conceptual translations of potential pseudogenes and to determine the number of disruptions (frame shifts, early stop codons and in-frame deletions).
Subsequently, NHEJ repair leads to target gene disruption by frame shift.
This includes 147 SNPs and 131 indels which result in premature stops, splice-site disruptions and frame shifts.
(iii) Realignment to remove spurious frame disruptions: Spurious frame disruptions can arise as alignment errors when comparing a protein to a nucleotide sequence [ 3].
*** The ratio stands for 'the number of frame disruptions not disrupting a protein structure domain assignment versus the number that do'.
Do the frame disruptions in these mRNAs avoid disruption of protein structure domains?
Alternatively spliced TCR mRNA induced by disruption of reading frame.
How frequent is genuine frame disruption in human mRNAs?
Our results indicate largely negative selection pressures related to frame disruption during gene evolution.
In this case, the net shift is 0 for subsequent exons and frame disruption is limited to a local region.
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