Sentence examples for disruption cells from inspiring English sources

For mechanical disruption, cells were resuspended in 200 µl of SDS sample buffer. 1 volume of glass beads was added, and the samples beaten in a Precellys 24 bead beater (Stretton Scientific Ltd., 3 cycles of 30 seconds beating, 30 seconds pause).

For disruption, cells were thawed, supplemented with 1 mM phenylmethanesulfonyl fluoride (PMSF), and sonicated well until the solution cleared completely.

For disruption, cells were initially shock-frozen in liquid nitrogen and then defrosted in a thermomixer (Eppendorf, Hamburg, Germany) at 1400 rpm at 30°C for 10 minutes.

To cure Δ cpaAIR disruption cells of the pSYCP-cpaAIR intron donor plasmid, a single Δ cpaAIR disruption colony was heat-shocked in 10 ml 2 × YTG medium without selection.

To measure levels of Δψm disruption, cells were harvested, washed twice with PBS, and then incubated with 20 nM 3,3'-diethyloxacarbocyanine (DiOC6; Molecular Probes, Eugene, OR, USA) for 15 min at 37°C.

To further confirm the induction of senescence after centrosome disruption, cells were also depleted of Cep192, a centrosomal protein which is a major regulator of pericentriolar material recruitment, centrosome maturation and centriole duplication that has been shown to be important for the centrosomal localization of NEDD1 and γ-tubulin.

Ugrinova, I. et al. Inactivation of nucleolin leads to nucleolar disruption, cell cycle arrest and defects in centrosome duplication.

After mechanical disruption, cell debris was pelleted by centrifugation (3000 rpm, 20 min, 4 °C) and the membranes were isolated from the supernatant by ultra-centrifugation (205,000 × g, 3 h, 4 °C).

After cell disruption, cell debris was removed by centrifugation (21,000 × g for 20 min).

Furthermore, the effects of magnetic labeling on the biological properties of A549 lung cancer cells, including cell viability, proliferation capacity, cytoskeletal disruption, cell cycle, and apoptosis, were investigated separately.

Injury of myofibers leads to rapid necrosis associated with sarcomere disruption, cell permeabilization, and proteolysis, which induces an acute inflammatory response characterized by the invasion of inflammatory cells (Brunelli and Rovere-Querini, 2008).