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Although both the soluble and immobilized peptides demonstrated membrane disruption capabilities, the latter showed a slower rate of kill, presumably due to reduced diffusivity and flexibility resulting from conjugation to the polymer brush.
For example, when high cell disruption capabilities were observed in experimental runs 15 20 (CDR >70%), high concentration of HBsAg (> 28 mg/L) were obtained.
However, although high cell disruption capabilities was observed in experimental runs 5 10, lower levels of HBsAg concentration were detected (8.65-15.10 mg/L).
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The highest disruption capability was achieved at CDR 78.96% of the amount of cells disrupted in run 20 and the lowest capability of cell disruption was observed in run 3 with CDR of 27.64%.
Subsequent passes would treat more resistant cells, resulting in lower cell disruption capability [ 35].
Additionally, a non-linear correlation between cell disruption capability and the specific protein release was observed for cell disruption process.
Thus, it was concluded that measurement on cell disruption capability alone would not sufficiency evaluate disruption efficiency.
The F-value for both cell disruption capability (11.53) and specific protein release (74.01) indicates that the model is significant.
Vice versa, in experimental runs 1 4 and 13, low cell disruption capability (CDR <55%) results in low recovery of HBsAg.
This indicates that pulse pressure was very important for maintaining the optimal level in the recovery of HBsAg while achieving the best possible cell disruption capability.
Low pure errors for cell disruption capability (22.87) and specific protein release (7.87) indicate a good reproducibility of the experimental data.
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