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The mouse and rat were found to have independent deletions disrupting use of the exon homologous to canine exon 27.
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Carvana is a new site on the scene disrupting used-car sales.
Cells were thawed and disrupted using a high-pressure cell disruption system from Constant Systems Limited (United Kingdom).
H3mm7 genomic loci were disrupted using the CRISPR/Cas9 system.
Bacteria were disrupted using a French press before centrifugation at 12,000 g for 90 min at 4 °C.
Bacteria were disrupted using a French press before centrifugation at 12000 g for 90 min at 4 °C.
Tissue samples were manually disrupted using a Tissue Lyser II (Qiagen) mixing 2 × 10 s at 20 s−1.
Cells were disrupted using a constant flow cell disruptor, and cell debris and inclusion bodies were removed by centrifugation at 10,000 g for 30 min.
The pellet was resuspended in buffer A (to which 1 mM EDTA was added) and cells were disrupted using a cell disruptor (Constant Systems).
The cell suspension was disrupted using Constant Cell Disruption Systems Constant Systems Ltd , Warwick, UK).
Cells were disrupted using a glass homogenizer and plated.
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