The final pellet was resuspended in 0.5 ml isolation buffer and the cells were disrupted in a nitrogen cell disruption bomb (model 4639, Parr Instruments, IL) at 1200 psi for 10 min at 4°C.
"Many middle-class families will have their lives disrupted in a significant way".
Prepulse inhibition (PPI) of the startle reflex is disrupted in a number of developmental neuropsychiatric disorders, including Tourette syndrome (TS).
Asteroid families are formed when an asteroid is disrupted in a catastrophic collision, the members of the family thus being pieces of the original asteroid.
When a star gets close to a super-massive black hole (SMBH), it can be disrupted in a flare that lasts anywhere between a few months to a few years.
The tissue is usually minced, blended, or otherwise disrupted in a medium that is suitably buffered to maintain the normal acid base balance of the tissue, and contains the ions required for many life processes, chiefly sodium, potassium, and magnesium.
To test the biological functions of the YABBY-binding sites in the CRM4, all three YABBY-binding sites were disrupted in a full-length YUC4 promoter (pYUC4), yielding pYUC4m.
The characteristic co-localization of TDRD1, 6 and 7 was disrupted in a mutant of mouse vasa homologue/DEAD box polypeptide 4 (Mvh/Ddx4), which encodes another evolutionarily conserved component of nuage.
By definition, people with chronic diseases have ongoing medical needs that can easily be disrupted in a disaster situation.
Last but not least, a total of around 9% of parameters were disrupted in a meta-analysis (Table 2).
The cells were disrupted in a cell homogenizer, and the insoluble fraction was removed with centrifugation at 23,000 ×g at 4°C for 1 h.
