Sentence examples for disrupted gently from inspiring English sources

Digested lungs were disrupted gently by passage through a 70-µm pore size cell strainers; residual RBCs, if any, were lysed by ACK buffer.

The digested lungs were disrupted by gently pushing the tissue through a cell strainer (100 µm, BD Biosciences).

Then the airway was cut open and the endothelium was disrupted by gently stripping the luminal surface with a blade.

D562 cells were detached by treatment with 0.025% trypsin-EDTA (Gibco Laboratories, Grand Island, NY, USA), and cell clumps were disrupted by gently pipetting up and down.

To disrupt cells gently, the gel plugs were transferred into Buffer B (0.2% sodium deoxycholate, 0.5% Brij 58, 0.5% sarcosine, 50 mM Tris-HCl pH 8.0, 100 mM EDTA pH 8.0, 0.40 M NaCl) and incubated at 37°C overnight.

Cell membranes were gently disrupted by nitrogen cavitation (25 pounds per square inch, 8 min) achieving disruption of 85% of cells.

The tissue was gently disrupted to release hepatocytes and the cells were centrifuged at 29 X G for 3 min at 4°C and washed two times in HBSS.

In group 1, the tissue was dissected with conventional scissors or gently disrupted with other instruments.

The mammospheres were gently disrupted by pipetting and resuspended in matrigel solution.

Briefly, we gently disrupted the tissue by collegenase I and IV, and randomly selected single cells from the tumor tissue and normal adjacent tissue.

Briefly, cells (10) were harvested, washed in PBS, pelleted, resuspended in homogenization buffer (0.25 M sucrose and 10 mM Hepes pH 7.4) and gently disrupted by dounce homogenization.