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They were told to "disrupt using all means transport, transmissions and communications of the Germans".
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Cells were thawed and disrupted using a high-pressure cell disruption system from Constant Systems Limited (United Kingdom).
The cell suspension was disrupted using Constant Cell Disruption Systems Constant Systems Ltd , Warwick, UK).
Cells were disrupted using a glass homogenizer and plated.
The suspended cells were disrupted using a high-pressure homogenizer (APV, Denmark) at 900 1000 bar.
The cells were disrupted using a Shake Master Neo (Bio Medical Science) by shaking the tube three times at 1,500 rpm for 1 min at 1-min intervals.
Dried stems from each treatment were pooled and disrupted using a beadbeater at 1500 rpm for 3 min (Microdismembrator U, B. Braun Biotech Int., Meisungen, Germany).
Fresh mycelium was disrupted using Fastprep with glass beads and 400 μL DNA extraction buffer (0.1 M Tris HCl pH 8.0, 1.2 M NaCl, 5 mM EDTA).
Chlorella, Nannochloropsis, and Dunaliella species have been successfully disrupted using this technique (Chen et al. 2013a, b; Wang and Yuan 2014; Lenneman et al. 2014).
To prevent the degradation of l-tryptophan and 5-HTP, the tnaA gene of E. coli BL21 (DE3) was disrupted using the Red/ET recombination system.
Madison Reed's founder Amy and her investors believe there is a market to be disrupted using technology.
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