The insertion of a CREE can generate a promoter at either its the 5' [ 36] or 3' end [ 37] and can disrupt the sequence into which it has been inserted.
For two IBD haplotypes separated by m meioses, looking along the chromosome, recombination ends the IBD at rate m times the recombination rate, while new mutations disrupt the sequence identity at rate m times the mutation rate.
To evaluate their potential roles as genetic switches, we constructed a series of isogenic strains, including LT2 mutLT2, LT2 mutLL3, LT2 mutLL4 and LT2 mutSL (Table 1), in which at least one base in the repeats within mutL or mutS was changed to disrupt the sequence identity among the repeats.
Mutational analyses showed that restriction was invariably lost when changes designed to disrupt the sequences responsible for multimerization were introduced.
However, as discussed in Washietl et al. [22], a stronger shuffling algorithm disrupts the sequence enough to not be a meaningful control.
This deletion disrupted the sequence into two small photolyase ORFs, which correspond to the CPD-DNA and FAD-binding domains of photolyases, respectively.
The rat (BN/SsNHsd) showed 80% identity for the first 59 bp of the sequence (Additional file 1, Figure S4), but a 271 bp deletion, relative to the mouse, disrupts the sequence (Additional file 1, Figure S5).
This 1-bp deletion mutation disrupted the sequence into two small ORFs, labelled phr-1 (Clbi58) and phr-2 (Clbi59) on the basis of their position in the ClbiNPV genome relative to the polyhedrin gene.
This deletion disrupts the sequence into two small photolyase ORFs, designated Clbi phr-1 and Clbi phr-2, which correspond to the CPD-DNA photolyase and FAD-binding domains of photolyases, respectively.
The analysis of motif alterations revealed eight significant associated Indels in COMT introns disrupting the sequence of five motifs, which represent binding sites for transcription factors RAV1, GAmyb, and DOFs 1, 2, and 3. DOF-type transcription factors are well known for their function as transcriptional activators or repressors of tissue-specific gene expression.
The non-linear cross-linking could disrupt the peptide sequence or presentability, thus lowering the efficacy and size of those AuNVs.
