Mutations designed to disrupt the pairing of a stem and loop structure, in which the uAUG codon is localized, reduced recognition of this codon, while complementary mutation restored a great extent of the recognition.
When a mutation occurs at a paired site and disrupts the pairing ability, a second compensatory mutation on the corresponding paired site may restore the pairing ability [ 25, 26].
The confusion had nothing to do with another judging scandal, like the one that disrupted the pairs event at the last Olympics, or with the much-maligned interim judging system.
Gatland said he expected the recall of the flanker Schalk Burger was an attempt by South Africa to disrupt the centre pairing of Jamie Roberts and Brian O'Driscoll which has been the Lions' most potent weapon this tour.
We confirmed that predicted miR-30e and -181d target sites in HtrA1-3′ UTR are critical for repression of HtrA1 expression by introducing point mutations that disrupt the base-pairing of both predicted target sites into Rluc-HtrA1-3′UTR construct.
In particular, large INDELs could disrupt the base pairing that occurs during the exome capture procedure, which would then result in insufficient coverage in those regions.> -wrap-foot>> We also calculated the validation rates of large INDELs (>5 bp) in each category.
Therefore, genotype A with C1858 rarely circulates as an HBeAg-negative mutant because 1896A would disrupt the preexisting C G pair and impair HBV genome replication.
It has been shown that for fully paired DNA three-way junctions, coaxial stacking of two helices is not possible without disrupting the base pairing, so fully paired junctions form only nonstacked conformations.
Five of the mutations disrupt the predicted base pairing pattern of the GLS (Fig. 3) and according to mFOLD (http://mfold.bioinfo.rpi.edu/) are sufficient to destabilize the wild-type structure (data not shown).
These lesions feature alkyl groups that disrupt the Watson Crick base-pairing interactions, thus facilitating their recognition by AlkB and its homologues in both ssDNA and dsDNA.
