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Mechanical cell dissociation was performed using Medicon disposable chambers (BD bioscience, USA).
To an economical point of view and taking into account the value of a meticulous technique, cytocentrifugation with disposable chambers remains the technical standard for current treatment of urinary samples.
Concerning cost-efficacy comparisons, it has been shown that the monthly cost of the two most efficient methods (Cytocentrifugation with disposable chambers and Cytyc Thinprep LBC) is strikingly different: there is a 92.8% to 154.5% increased cost for LBC versus cytocentrifugation with disposable Megafunnels and Cytofunnels, respectively [ 19].
The data show that 1) smearing allows good overall results to be obtained, 2) Cytocentrifugation with reusable TPX® chambers should be avoided, 3) Cytocentrifugation using disposable chambers (Cytofunnels® or Megafunnel® chambers) gives excellent results equalling or surpassing Thinprep and 4) Millipore filtration should be avoided, owing to its poor global quality.
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Compared to disposable observation chambers, our chamber uses high quality coverslips permitting a better adjustment of the objective correction collar.
Compared to disposable plastic chambers and cultured dishes used for in vitro tests, MEAs are typically reused in experiments since they are expensive.
Before or during each experiment, parasite density was microscopically determined every 24 hours using Uriglass disposable count chambers (Menarini diagnostics) to follow up the growth and differentiation profile.
This cell suspension was cytospun (Cytospin 3; Shandon Scientific, Cheshire UK) using Shandon Megafunnel disposable sample chambers.
A single technician scored sperm concentration in duplicate using disposable counting chambers (Cellvision, Heerhugowaard, the Netherlands), and determined sperm morphology on air-dried Papanicolaou-stained seminal smears.
A disposable cell chamber (DCC) was a closed chamber with a total volume of 4.65 ml.
A miniaturised bioluminescence sensing system, which would allow sensitive and continuous monitoring of ATP, with an integrated and low-cost disposable microfluidic chamber for handling of biological samples, is highly desirable.
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