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Mechanical cell dissociation was performed using Medicon disposable chambers (BD bioscience, USA).
To an economical point of view and taking into account the value of a meticulous technique, cytocentrifugation with disposable chambers remains the technical standard for current treatment of urinary samples.
The data show that 1) smearing allows good overall results to be obtained, 2) Cytocentrifugation with reusable TPX® chambers should be avoided, 3) Cytocentrifugation using disposable chambers (Cytofunnels® or Megafunnel® chambers) gives excellent results equalling or surpassing Thinprep and 4) Millipore filtration should be avoided, owing to its poor global quality.
Concerning cost-efficacy comparisons, it has been shown that the monthly cost of the two most efficient methods (Cytocentrifugation with disposable chambers and Cytyc Thinprep LBC) is strikingly different: there is a 92.8% to 154.5% increased cost for LBC versus cytocentrifugation with disposable Megafunnels and Cytofunnels, respectively [ 19].
Compared to disposable observation chambers, our chamber uses high quality coverslips permitting a better adjustment of the objective correction collar.
A disposable cell chamber (DCC) was a closed chamber with a total volume of 4.65 ml.
A miniaturised bioluminescence sensing system, which would allow sensitive and continuous monitoring of ATP, with an integrated and low-cost disposable microfluidic chamber for handling of biological samples, is highly desirable.
To measure dynamic surface tension, a test solution was loaded into a disposable sample chamber, within which a spherical air bubble was formed that maintained contact with outside air.
MCP-1 (Peprotech, London, UK) was diluted in Gey's Balanced Salt Solution (GBSS) with 1% BSA to a final concentration of 12.5 ng.ml−1; 29.2 µl was added to each well of 96 well disposable chemotaxis chamber (Neuroprobe, Gaithersburg, USA) and an 8 µm polycarbonate filter membrane fitted.
Spores present in the homogenised solution were subsequently counted using a "Fast Read 102" disposable counting chamber (Biosigma, EU).
The two liquid phases were extruded through a disposable dual chamber applicator and then sprayed through the applicator with an inert gas carrier to form a thin, uniform film of fibrin glue covering the wound.
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