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The results support that the WPAI-GH displays construct validity as measured by moderate to strong correlations with all health status measures in terms of functional disability, pain, fatigue and disease activity (Table 3).
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In the first display construct, Avd was produced solely as a fusion with pIII (Avd-pIII; Figure 1A), whereas in the Avd/Avd-pIII display construct, free Avd subunits were produced in addition to the pIII fusion.
Yeast displaying 202 08 intein fusion proteins were reacted with MESNA to release the scFvs or GFP from the display construct, thereby generating scFv- and GFP-thioester proteins.
Reaction with a nucleophile, such as MESNA, releases 4 4 20 from the intein and the yeast display construct while simultaneously appending a carboxy-terminal thioester onto 4 4 20.
The retention of 202 08 catalytic activity was confirmed by examining the relative amount of 4 4 20 cleaved from the yeast surface display construct in a MESNA release reaction.
We present evidence that the AQoL-6D domains and factor scores depicted here display construct validity and are interpretable over a range of community contexts.
The CPAx score at ICU discharge has displayed construct validity by crudely discriminating between groups with different functional needs at hospital discharge.
Western blotting with an anti-FLAG antibody demonstrated nearly quantitative release of each of the scFvs and GFP from the 202 08 fusion display construct.
The ability to release the scFvs and GFP from the display construct in an intein-dependent manner was also evaluated as previously described.
If the CPAx is to display construct validity, then higher CPAx scores at ICU discharge should be associated with better functional outcome in terms of rehabilitation/care needs at hospital discharge, crudely defined by discharge location.
B) Four-sided virtual image display constructed in Second Life.
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