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This is illustrated by a mutant generated using dithitotreitol as an egress enhancer, which displays a defect in dithitotreitol induced egress but not in Ca2+ ionophore induced egress.
Deletion of C. neoformans STE12 displays a defect in filamentous growth during the monokaryotic fruiting process and no role has been previously noted regarding invasive growth [59], [60].
PRO22, the homologous gene product of S. macrospora, also contributes to hyphal fusion events and the corresponding mutant fails to develop mature fruiting bodies and displays a defect in ascogonial septum formation (Bloemendal et al. 2010; Rech et al. 2007).
Our results are consistent with the idea the S288c-derived lab strain displays a defect in Mig3p-dependent signaling, since there were no genes whose expression was affected by MIG3 deletion in the lab strain, either under standard or ethanol-stress conditions (File S1).
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Wnt5a−/− mice also displayed a defect in (mid brain morphogenesis, including an impairment in midbrain elongation and a rounded ventricular cavity.
Additionally, when comparing HBeAg positive with HBeAg negative patients, we found that especially HBeAg-positive patients displayed a defect in the CpG-induced IFNα production (Fig. 6BD).
To the best of our knowledge this represents the first mammalian transgenic model displaying a defect in mitotic chromosome condensation and is also the first mouse model for impaired Mcph1-function.
We have no explanation for the fact that so few mutants identified by us display a defect linked to Sonic hedgehog signaling, as was the case in the screens mentioned above.
Such mutants displayed a defect in various cell wall functions and biosynthesis, Mg2+ transporter, pore-forming protein, and other membrane-bound proteins suggesting that the cell envelope clearly plays an important role in M. tuberculosis resistance to acid and maintenance of the intrabacterial pH.
D6-deficient macrophages display a defect in conversion to a resolution phase macrophage.
In the spinal region, embryos displayed a defect in PNP closure.
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