Consequently, MCF-10A cultures, which had been exposed to dox and displayed invasive behavior, were subsequently cultured in medium lacking dox.
Secondly, the acinar structures, in which B-RafV600E has been induced, display an invasive behavior after a few days in that sense that individual cells protrude into the matrigel and thus most likely through the basement membrane, which is deposited from day 4 onwards [ 1].
Six of the 23 patients displayed invasive disease and, consequently, underwent radical resection.
Most of tumors show retarded growth, but a small portion also display invasive growth.
Gliomas are a type of primary brain tumor that display an extensive invasive behavior, but do not metastasize.
We labeled MDA-MB-231 breast cancer cells which normally do not display a highly invasive behavior with a fluorescent dye and we then embedded a monolayer of these cells in collagen.
We found that MCF-7/DOX breast cancer cells displayed enhanced metastatic and invasive behavior both in in vitro cell invasion assays and in vivo in a mouse lung tumor model.
For example, tamoxifen-resistant MCF-7 breast cancer cells (MCF-7R) display enhanced motile and invasive behavior as well as accompanying EMT-like properties compared to the parental MCF-7 cell line in vitro [ 12, 13].
Our assay displays several advantages over previous invasion assays as it is allows the quantitative analysis of directional invasive behavior of tumor cells in a 3D environment mimicking the tumor microenvironment.
In GSE40059, cell lines were reassigned as less invasive or invasive mostly according to their phenotype, invasive behavior and expression of the EMT marker genes77,78,79,80.
Hoshino, D. et al. Exosome secretion is enhanced by invadopodia and drives invasive behavior.
