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To the best of our knowledge this represents the first mammalian transgenic model displaying a defect in mitotic chromosome condensation and is also the first mouse model for impaired Mcph1-function.
female fertile-1 (ff-1) was originally isolated as a mutant displaying a defect in female fertility.
However, when we expressed the single Y120A and P121A HCCS mutants alone (i.e., in the absence of the cytochrome c acceptor), we discovered that both mutants exhibited heme-binding defects, with the former displaying a defect nearly as severe as the Y120A/P121A HCCS double mutant.
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This is illustrated by a mutant generated using dithitotreitol as an egress enhancer, which displays a defect in dithitotreitol induced egress but not in Ca2+ ionophore induced egress.
Wnt5a−/− mice also displayed a defect in (mid brain morphogenesis, including an impairment in midbrain elongation and a rounded ventricular cavity.
Additionally, when comparing HBeAg positive with HBeAg negative patients, we found that especially HBeAg-positive patients displayed a defect in the CpG-induced IFNα production (Fig. 6BD).
We have no explanation for the fact that so few mutants identified by us display a defect linked to Sonic hedgehog signaling, as was the case in the screens mentioned above.
Such mutants displayed a defect in various cell wall functions and biosynthesis, Mg2+ transporter, pore-forming protein, and other membrane-bound proteins suggesting that the cell envelope clearly plays an important role in M. tuberculosis resistance to acid and maintenance of the intrabacterial pH.
D6-deficient macrophages display a defect in conversion to a resolution phase macrophage.
In the spinal region, embryos displayed a defect in PNP closure.
mir-83 (n4638 ); mir-34 (gk437 ) double mutants display a defect in gonad morphogenesis.
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CEO of Professional Science Editing for Scientists @ prosciediting.com