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Positive immunostaining was localized throughout each division (cervical, thoracic, lumbar and sacral) of the spinal cord; however, regions immediately adjacent to the central canal of the spinal cord or to the subarachnoid space surrounding the spinal cord displayed the highest intensity of staining (Figure 1a, S6).
The laser power was adjusted so that the signal intensity was below saturation for the developmental stage that displayed the highest intensity and all subsequent images were scanned at that laser power.
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The SERS spectra obtained after a heating time of 1 min displayed the highest relative intensity at 1362 cm−1.
The majority of outer root sheath (ORS) follicular keratinocytes co-expressed C/EBPα and C/EBPβ in their nuclei as did the vast majority of sebocytes of the sebaceous gland which displayed the highest levels of staining intensity for C/EBPα and C/EBPβ (Fig. 1B).
The 500 segments displaying the highest signal intensities were then added back into the data.
The image files given by the Typhoon reader software are automatically adjusted to best display the bands with the highest intensity.
This is consistent with the high intensity of infection displayed in the blood of nestlings, as compared to the reduced intensity of infection in adults (Fig. 3).
Where is the high intensity there?
The high intensity part includes the jumping rope.
As with the data shown for rats, GAD67-GFP mice also displayed a higher intensity of immunofluorescence for the α7 nAChR subunit in the inner third of the granule cell layer and subgranular zone than in the outer two thirds of the granule cell layer of the DG (Fig. 2C).
In one set of experiments, the naïve males of the Mather, California, strain displayed significantly higher intensity of courtship, as measured by frequencies of wing vibration and attempted copulation, and mating success than the experienced males in the first mating assays.
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