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At 4ss, when CNCCs are mainly premigratory, PGK Cre Dbx1DTA embryos displayed a strong reduction in sox10 staining whereas Triple-Cre Dbx1DTA Triple-Cre Dbx1DTAignificantly lembryosected (Fig. 8L–N).
Cells that lack stam displayed a strong reduction of the argos expression, when clones of mutant cells were located in the vein tissue (Fig. 6B) Although stam2L2896, hrs mutant clones are tiny and do not permit to analyse argos expression, we also found a strong reduction of argos in vein cells carrying simultaneously the stam2L3297 and hrsD28 mutations (Fig. 6C).
We found that, in contrast to the other enhancers, the HCNR 81728 enhancer displayed a strong reduction of the area of GFP expression (expressed as % of GFP domain) after blockade of Hh pathway by CyA (Figure 4A C) suggesting that probably tbx1 and pou3f4 share the same regulatory mechanisms.
Real-time quantitative PCR results (Fig. 2b) allowed concluding that the atnup62 mutant displayed a strong reduction of the spliced transcript.
Notably, both p38−/− and p57−/− cells displayed a strong reduction on their ability to survive to osmostress when compared with wild type.
Immunocytochemistry on cardiac fibroblasts displayed a strong reduction in type I collagen staining in both Aga2 severe cells and ECM (Fig. 2A), and a slight decrease in collagen staining in Aga2 mild cells.
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Seedlings of hsfc1b subjected to mannitol displayed a stronger reduction of shoot and root growth than the Dongjin wild type (Fig. 4D).
They also examined octuple acs lines that displayed a stronger reduction in ACS8/ACS11 transcript levels, which were only able to be propagated as heterozygotes as the more severe disruption of the ACS genes resulted in embryo lethality.
mib embryos display a strong reduction in Notch signaling [13] and hes5, her4 and heyl were collectively shown to be reduced in mib mutant fish and/or mice [35], [36], [37], [38], [39].
Similar to results in bulk cells, clones lacking the upstream promoter region of MALAT1 do not display a strong reduction in RNA levels (Fig. 6a).
Later, Korsmeyer and colleagues generated a double knock out for both BAX and BAK displaying a strong reduction in the ER levels of Ca2+, as well as reduced cytoplasmic Ca2+ waves and mitochondrial uptake.
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