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New open termini in the region of residues 53 or 99 in two lichenase CP variants (CN-53 and CN-99) had no effect on their activity and thermal tolerance versus another variant CP variant, CN-140 (cut in the region of residue 140), which displayed a dramatic decrease in the activity and thermostability.
MdPIN1 transcript amount displayed a dramatic decrease in seeds whereas not much difference was observed in the other tissues.
Given that NCOA4I489A/W497A displayed a dramatic decrease in ferritin binding when expressed in cells, we next evaluated whether cells expressing this mutant undergo ferritinophagy upon iron depletion.
For instance, the temperature-sensitive strain complemented with the OLE1 promoter that upregulates OLE1 expression displayed a dramatic decrease in palmitic acid, as well as HSP expression (17).
Conversely, the rough variant (57B) displayed a dramatic decrease in the rate of transcription for the 9 selected genes, particularly those encoding peptide synthetase pstA, glycosyltransferase gtfA, the methyltransferases mtfB and mtfC, and the lipid transporters tmtpA, tmtpB, and tmtpC).
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Miner1 KO cells displayed a dramatic reduction in ER Ca2+ and profound mitochondrial Ca2+ loading.
Interestingly, mutant H547P displays a dramatic decrease in DA uptake, which is accompanied by decreased total DAT expression (data not shown).
The results showed that Huh-7/HBSP/B[alpha]P displayed a dramatic alteration in cell cycle phase distribution with a decrease in the fraction of the cells in G1 phase and a corresponding increase in the fraction of the cells in S phase.
Rs1 calvariae displayed a dramatic increase in bone volume with partial loss of cortical structure.
PTX-mPEG-PCHLG-Nps were spherical and well-dispersed and displayed a dramatic sustained-release property.
Triple KO cells displayed a dramatic cell division defect.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com