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The results show that these PNAs display uptake similar or even superior to that of the peptide alone and PNA peptide conjugate, as well as good anti-miR properties.
Indeed, both are high-affinity transporters (KM of 25 μM for ExuT versus 1.2 μM for GAT-1), display uptake rates in a very similar range (Vmax of 38 nmol min-1 mg protein-1 for ExuT versus 12.8 nmol min-1 mg protein-1 for GAT-1), transport D-GlcA in addition to D-GalA and are sensitive to the presence of uncouplers [ 35].
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The functional Gap1 mutant proteins displayed uptake activities ranging from 25% to 120% of the activity of native Gap1 (Table 2 and data not shown).
In the presence of choline chloride, WT cells took up 6.9 ± 0.3 nmol of Pi (mg of protein)−1, whereas all mutants and control cells displayed uptake levels below 5 nmol of Pi (mg of protein)−1 (data not shown).
PET series were converted to display Standardised Uptake Values (SUV) adjusted to the body weight of the animals and merged with CT sets.
(C) and (F) display the uptake in the liver, kidney and lung at early (9 to 11 min) and late (55 to 60 min) time points after injection of (R -[11C]PAQ oR -[11C]PAQC]PAQ in rat and morse, Respectively.
These compounds should display increased uptake and clearance similar to that observed for our ABPs.
Most notably, these PS-dependent recognition patterns are realized in vivo whereby alveolar macrophages display enhanced uptake of PS-coated SWCNT during an inflammatory pulmonary response induced by aspiration exposure.
Plants suffering from water-deficit often display reduced uptake of mineral nutrients.
The carboxyl groups present the property to be negatively charged and therefore display rapid uptake in lymphatics and excellent retention in lymph nodes [ 12, 21, 24].
Taken together, our data demonstrate that macrophages alternatively activated by IL-13 have an overall increased capacity of OxLDL clearance, as they display increased uptake as well as increased cholesterol efflux capacities without enhancing foam-cell formation.
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