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Our results showed that we were able to generate, by a phage display procedure, mimotopes with sequence motifs PGxI/LN and CxPDDSSxC, and to identify them as functional motifs of the Pet, Pic and other SPATEs involved in their biological activities.
The differential display procedure was performed as previously described [ 15- 18].
The differential display procedure and other analytical steps were developed with R [ 47].
VN developed the algorithm for the differential display procedure, participated in the data analyses and reviewed the manuscript.
VN developed the algorithm for the differential display procedure, processed the SAGE data, participated in the data analyses and reviewed the manuscript.
We thank Randy Kerstetter for suggesting the modified transposon display procedure to characterize Ds insertion sites in W22 and Yubin Li, Qinghua Wang, and Jun Huang for valuable comments on the manuscript.
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We have even undertaken the extraordinary step of commissioning an independent audit of our flight display procedures.
Noteworthy, for discovery of tumor-associated molecules, it may be beneficial to use a combination of various digital differential display procedures and experimental data on gene expression.
All participants were informed of the stereo video and ROI images displaying procedure for each sequence, shown as Figure 9.
More specifically, the genome complexity reduction step was achieved thanks to a MITE-display procedure which utilizes the Ae. aegypti Pony element [ 15] as an additional primer anchor.
The αRep scaffold, the library construction and the phage display selection procedure against the biotinylated target EGFP were previously described [ 26].
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