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Diezmann, F. & Seitz, O. DNA-guided display of proteins and protein ligands for the interrogation of biology.
Phage display involves the combinatorial display of proteins or peptides on the surface of bacteriophage.
We demonstrate that this strategy enables the display of proteins on 2D and 3D surfaces without compromising protein function through direct cross-linking or physisorption.
Since for many biological signals and matrix proteins such mimetic peptides are not available, this approach can also be adopted for the display of proteins by modifying the protein with a DNA tether, as exemplified in Supplementary Figs 10 and 11.
We expect that the relative plasticity exhibited by ClyA as a fusion partner should prove useful for: (i) further mechanistic studies to identify the vesiculation machinery that regulates OMV secretion and to map the intracellular routing of ClyA-containing OMVs during invasion of host cells; and (ii) biotechnology applications such as surface display of proteins and delivery of biologics.
The zeta based plasmid employs the C-terminal end of the YlCWP1 for cell surface display of proteins.
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The selectivity of these PNAs could influence the design of new quadruplex-targeting molecules or allow the quadruplex structure to be used as a scaffold for multivalent display of protein binding ligands.
It is designed for easy and rapid image manipulation and display of protein molecules, and is intended for users who need to quickly produce high-resolution images of protein molecules but do not have the time or inclination to use a software molecular visualization system.
These results demonstrate the successful display of protein E using Non-OmpA as an anchoring motif.
Several mechanisms for the surface display of protein include covalent interactions with long-chain fatty acids and covalent or non-covalent interactions with the cell wall through linkage to the cell membrane or via transmembrane domains (Desvaux et al. 2009).
The filtering plug-in toggles the display of protein annotations.
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