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Functional proteins fused with an anchor protein, Mms13, have been demonstrated to be an effective and stable method for the display of functional proteins on BacMPs.
For coregistration and display of functional data, high-resolution 3D data sets (1 × 1 × 1 mm) were acquired for each individual using a T1-weighted sequence.
This differs from the incorporation of foreign proteins or glycoproteins into the baculoviral envelope without fusion to GP64, such as the envelope incorporation and display of functional human beta-2 adrenergic receptor (ß2AR) described in an earlier study [ 54].
Comparing both tag orders, the Myc-His order (pHAL30) results in improved production of functional scFv compared to His-Myc (pHAL14) whereas the display of functional scFv on phage is not effected.
We assume that the oligomerization of Avd, secreted by the pelB signal sequence, occurs in the periplasmic space of E. coli, allowing the display of functional Avd on the phage, as is the case for the display of the antibody Fab fragment, which requires folding of the heavy and light chains for the assembly of a functional antibody molecule [ 24].
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Reconstruction and display of functional-anatomic fusion images were performed on the Syngo MI workplace (Siemens Healthcare, Forchheim, Germany).
They also suggested that N18E2 could function as a universal N-terminal signal peptide for BV-display of functional scFv of biological interest.
In addition, expression of soluble Fab molecules with antigen-binding reactivity, recombinant phage titer and display level of functional Fab-ΔpIII on the phage progenies of the DVS-II were comparable with a conventional phage display system using a single phagemid vector.
A prerequisite for a good up to date antibody gene library is a phagemid that allows both, a high display level of functional antibody fragments on phage and a good expression of soluble antibodies for screening.
Following the functional scans, a T1-weighted structural image (1 mm resolution) was acquired for coregistration and display of the functional data and for the voxel-based morphometry (VBM) analysis.
Following the functional scans, a T 1-weighted structural image (1 mm resolution) was acquired for coregistration and display of the functional data.
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