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In comparison with Non-OmpA, weak fluorescence signals which were observed in E. coli cells containing Lpp-OmpA plasmid, suggesting lower surface display efficiency.
But battery density has been improving very slowly over the last few years, and advances have had to be in processor and display efficiency, in order to better use that limited store of power.
Herein, we comprehensively summarize the variant factors affecting lipase whole cell catalyst activity and display efficiency, including the structure and size of target proteins, screening anchor proteins, type and chain length of linkers, and the appropriate matching rules among the above-mentioned display units.
Since both too low or too high transcription levels decrease display efficiency [41], [43], we tried to optimize the expression level of the originally used pTac promoter by inducing it at various IPTG concentrations.
We compared display efficiency of scFv antibodies in gpD and gpV-fused phages.
Finally, both phage display efficiency and yield of soluble Fab fragments were analysed.
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All PCRs displayed efficiency between 95% and 103% and every reaction was optimized to keep the difference of the amplification efficiencies between the single special gene of the plasmid and chromosomal aiiA less than 2%.
Couples of primers were designed on different exons using Primer 3 software to avoid DNA amplification (Table S1) and for each couple of primers, all qPCRs displayed efficiency between 90% and 110%.
All PCRs displayed efficiencies between 94% and 100%.
All PCR reactions displayed efficiencies between 87 and 115%.
For genes displaying efficiencies different from 2 (E ≠ 2), Ct values were adjusted according to the model described by Steibel et al. [ 24].
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