Furthermore, this novel display cassette is also suitable for the hydrolyzation of insoluble substrates including lignocellulosic biomass.
The display cassette of pDJ01 contains the promoter p psp, followed by the ribosome-binding site, the start (ATG) codon, multiple cloning site and the sequence encoding the C-domain of phage protein pIII.
We demonstrated that the combinations of a promoter and an anchoring domain in the display cassette had significant effects on the efficiency with which the enzyme integrated into the cell wall.
The cell-surface display cassette, which includes the I2 region (the 3′ non-coding region between gene YFL021W and YFL020C, used for integration), LEU2, SED1 promoter, SED1 anchoring region, and SAG1 terminator was amplified from plasmid pIL2GA-SS using primers P-EG2 and EG2-A.
Our dual-display cassette system approach also allows for easy exchange of both the anchor protein (SBP) and the displayed biomarker.
These two cassettes display essentially the same structure with a small overall r.m.s.
The combination of T7 phage display and trinucleotide cassette randomization resulted in a novel resource for the potential isolation of binding peptides for new and previously studied molecular targets.
The myosin-X MyTH4 FERM cassette displays a rather elongated shape with multiple globular domains.
However, in the case of the A. macleodii strains studied here each cassette displayed independent regulation.
