Sentence examples for display and selection from inspiring English sources
Strategies include both in vivo and in vitro methods and embrace the latest concepts for antibody display and selection.
Ribosome display and selection has the potential to generate and display large libraries more representative of the theoretical optima for naı̈ve repertoires (1014).
We conclude that the pIX coat protein complements other display systems in filamentous phage as an efficient vehicle for low copy display and selection of Fab proteins.
These may both complement current systems and be used as alternative scaffolds for display and selection to further improve phage display as the ultimate combinatorial engineering platform.
We discuss the application of ribosome display and selection in conjunction with variable domain (CTLA-4) libraries as the first step towards this objective and review affinity maturation strategies for in vitro ribosome display systems.
However, C-terminal fusion might allow efficient display and selection for some antigenic C-terminal protein domains.
Phage display panning and selection of mimotope-immunized mouse spleen-derived antibody Fab library showed that HeLa cell-reactive Fabs were successfully retrieved from the library.
Although we did not achieve our primary goal, we showed that phage display panning and selection against native antigen-expressing cells could obtain conformation-specific antibodies, which are, in general, difficult to select by peptide immunization and hybridoma technology.
Meanwhile, systems based on virus-like particles (VLPs) permit the engineered high-density display of specific epitopes but are incapable of peptide library display and affinity selection.
Here we describe a phagemid system for the efficient display and affinity selection of Fabs through linkage to the minor coat protein pIX.
An improved procedure involving bivalent display and stringent selection against a model target, Her2, led to a 107-fold enrichment of a DARPin (H10-2-G3, known to bind Her2 with picomolar affinity) over a non-binding DARPin after three rounds of selection.
