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This vector allows both antibody phage display and expression of soluble antibody fragments due to an amber stop codon between the antibody coding region and gIII.
It seems that scFvs exhibiting a high level of display and expression often harbour a VK1 derived VL domain and those exhibiting low expression and display tend to harbour a VH3 derived VH domain.
At the nucleotide level, the scFv sequences are similar (See additional file 2) and the CAI covers a small range, suggesting that minor differences in codons could account for large differences in display and expression.
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In contrast, for displays whose development and expression require learning, a direct link with cognition is possible because of a shared dependence on brain function.
Nrtn and Gdnf are barely detected in DRG and SC at E13.5 and display increased expression in DRGs at E15.5.
Consistent with this hypothesis, AtNRP1 and AtNRP2 display similar expression profiles and subcellular localizations as the soybean orthologs [ 59].
Their private chef service, bigLITTLE Get Together, offers something close to walking through a museum gallery of curated colors, inventive displays and seasonal expressions of flavor.
As a control, conditions for imaging luminescence were tested on worms that carry the plasmid PTG96 [ 36] and display expression of GFP on its own.
Following neural induction, hATSCs changed toward neural morphology and displayed expression of MAP2 and GFAP.
Moreover, Cre transgenic mouse strains tend to display unspecific expression, and thus a knockout phenotype, in various cell types.
There are three Per isoforms in mice (mPer1, mPer2, mPer3) that display different expression and regulation patterns [41], [42].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com