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Western blotting analysis of constructs with internal HA epitope revealed that in whole cell extracts, with the exception of the one bound to C-terminal, it did not display a reduced level of protein accumulation.
Upon ER stress, both Ire1 ΔV mutant and full-length Ire1 protein display a reduced level of interaction with BiP.
In addition, healthy people playing borderline partners display a reduced level of theory-of-mind, as would be expected if the borderline partner were deficient in higher-order theory-of-mind terms (Xiang et al., 2012).
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A type of allele from which either the gene product is expressed at reduced levels relative to the WT or the gene product displays a reduced level of activity.
As Additional file 5: Table S1 indicated, these PR genes displayed a reduced level of expression at 72 HPI in APIP12-KD mutant.
As shown in Figure 2A, compared to TAP-stop, HeLa expressing TAP-Bcl-xL displayed a reduced level of the PARP cleavage product.
Conversely, cells overexpressing a V5-tagged Pdro displayed a reduced level of cellular free cholesterol content, as compared to parental cells (SHEP: 12.3±3.42 µg/mg protein; SHEP-Pdro-V5: 3.91±1.21 µg/mg protein. Values are mean ± SD. n = 3, p≤0.05 using Mann Whitney test).
Compared to these Vmax values, both YbaS and YneH displayed a reduced level of catalytic activity at pH 6.0.
12 month-old S7ΔEx1 mice further displayed a reduced level of neurogenesis based on the numbers of cells expressing doublecortin (DCX), a marker for newborn neurons.
It also indicates that although Fyn1 displays a reduced level of coupling between the SH3 domain and the ATP-binding site, enhancing the interaction between the SH2 domain and C-terminal tail is able to engage the SH3 domain and stabilize an αC helix-out inactive ATP-binding site.
For example, periostin null mice exhibit a significant decrease in the thickness of the collagenous dermal layer of the skin and display abnormal collagen fibrillogenesis and a reduced level of collagen cross-linking 10, 14.
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