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We measured the displacement of wild type cells during 15 minutes (see Fig. 6A), and fitted the observed mean square displacement to this equation, yielding a speed of S = 10.4+/−2.1 µm/min and a persistence time of P = 3.4+/−0.5 min (mean and 95% confidence; Fig. 6E,F).
DOI: http://dx.doi.org/10.7554/eLife.05560.015 We next compared the geranylgeraniol-mediated displacement of wild type and N102S UBIAD1 from reductase using co-immunoprecipitation.
Thus, we postulate that binding of UBIAD1 to geranylgeranyl pyrophosphate produced by phosphorylation of geranylgeraniol triggers displacement of wild type UBIAD1 from reductase, allowing the protein to translocate to the Golgi.
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The observed reduction in FRAP recovery times between the wild type and mutants demonstrates that a measurable assay window should exist for the displacement of wild-type BRD4 from chromatin by small-molecule inhibitors of the bromodomain.
In contrast to control clones, ptc mutant clones contained more labeled CySCs on average by 14 dpci and were often seen to take over the entire somatic lineage, presumably by causing the displacement of wild-type CySCs (Fig 2B, compare boxes in Fig 2C to those in Fig 1G).
In contrast, data for mean squared displacement of the wild-type motor is fitted with n=2, indicating directed movement.
In addition to impairing activity, some mutated forms of pol β can have additional consequences resulting from nonproductive binding of damaged DNA intermediates or the displacement of native, wild-type pol β from DNA repair complexes.
Thus, gain of Hh signaling results in niche colonization by the mutant cell, as a consequence of the displacement of resident wild-type CySCs and GSCs at the niche.
(D ) Mean-squared displacement analysis of wild-type Cik1 Kar3 at two different salt concentrations in the presence of ATP.
Even though invasion of the transgene did not lead to local extinctions (a result opposite to Muir and Howard's 1999, 2001 findings), it did result in genotypic displacements of the wild genotype by transgenic and/or hybrid genotypes with increasing sneakers' mating success when the transgene was additive, recessive or dominant.
This decrease was associated with a reduced number of receptors at the cell surface with no apparent changes in receptor affinity for the ligand as the percentage of 125I-insulin displacement at saturating unlabeled insulin concentrations was similar in both groups (58±13% and 62±8% displacement for wild type and dmpk−/− mice, respectively).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com