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DOI: http://dx.doi.org/10.7554/eLife.05397.009 We examined the possibility that CLR01 might simply displace ThT from fibrils by employing a ThT displacement assay (Lockhart et al., 2005).
Figure 1 Displacement assay.
We used fluorescence-quencher pair displacement assay upon progesterone binding for the determination of P4.
Complexation stability was evaluated using agarose gel electrophoresis and EtBr displacement assay.
Their stability was investigated by a heparin displacement assay as well as by DNAse I assay.
To screen for G4 ligands, we modified a G4-FID (G-quadruplex Fluorescent Intercalator Displacement) assay.
Also, the Wang ARS displacement assay [17] was modified and used for the electrochemical detection of saccharides [84].
In vitro integrin-binding of FPyPEGCBT-c RGDfK) was evaluated using a standard displacement assay oFPyPEGCBT-c RGDfKn.
Using displacement assay, we first identified aptamers which bind to different aptatopes on the surface of the toxoid.
Here we describe the design, development, and optimization of a continuous coupled fluorescence displacement assay to measure BioA activity.
The DNA binding characteristics of the carboxamides were evaluated by thermal denaturation experiments and by ethidium bromide displacement assay.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com