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This multimeric ssDNA then serves as a template for multiple reverse primers to hybridize, extend, and displace downstream DNA, generating a large ramified (branching) DNA complex.
This multimeric ssDNA then serves as a template for multiple reverse primers to hybridize, extend, and displace downstream DNA, generating a large ramified (branching) DNA complex, and resulting in an exponential amplification.
Phi29 DNA polymerase can displace downstream 5′-termini DNA strand to extend the growing 3′-termini strand by a simple branch migration reaction, and 3′-termini can be displaced as well in a similar way.
Phi29 DNA polymerase can displace downstream 5′-termini DNA strand to extend the growing 3′-termini strand (Chen et al., 2014) by a simple branch migration reaction, and 3′-termini can be displaced as well in a similar way.
Similar(56)
Sulfur poisoning of upstream NOx storage sites caused the active NOx-storage zone to be displaced downstream.
It can also be observed that the location of the peak in radiative heat flux with Russian semi-anthracite coal at 68% recycle is displaced downstream.
The increased areas of low wind speeds and displaced downstream far-field low wind speed (DFLWS) zone are the two important flow modifications that would have negative impacts on outdoor thermal comfort and air pollutant dispersion in built-up areas where TWPs exist.
Our results indicate that the nuclei of ECs can be displaced downstream under a direct action of hydrodynamic drag.
The reaction proceeds through a strand displacement mechanism with the phi29 DNA polymerase extending 3'-termini while concurrently displacing any downstream copies starting from their 5'-ends [ 1].
We were also concerned that the ssDNA labeling we observed might be the result of exonuclease-deficient (exo–) Klenow's strand displacement synthesis extending the 3′-end of a nick in the DNA and displacing the downstream DNA strand rather than the result of filling ssDNA gaps in the template.
Then, a DNA polymerase extends the bound forward primer along the C-probe and continuously displaces a downstream strand, generating a multimeric single-stranded DNA (ssDNA), analogous to in vivo 'rolling circle' replication of bacteriophage.
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