The proposed merger would mean the more-profitable Indian Super League franchises would enter the I-League, and displace I-League teams, that would be relegated to the second division, despite performance in the previous season, and then threatened with competition from deeper pockets.
The 'native' conformation (connectivity 1 3, 2 4) and the 'ribbon' fold (connectivity 1 4, 2 3) were both able to displace I-Bgt bound to Ls-AChBP.
These concentrations were much higher than those found to displace I-labeled IGF-1 from IGFBP-3 in the radiobinding assay.
The penetration of NBI-31772 into cartilage was measured by its ability to displace I-labeled IGF-1 from cartilage IGFBPs.
This experiment was set up to determine whether NBI-31772 would displace I-labeled IGF-1 from its IGFBPs within cartilage.
Thirdly, the significant drop in Bmax for I-CXCL10 binding at the D195N and E196Q mutants, coupled with the relatively poor ability of Cp#1 to displace I-CXCL10, leads us to omit Cp#1 from these experiments.
The ability of peptides to displace I-CGRP binding to SK-N-MC cell membranes was measured in a microcentrifugation-based binding assay, as described previously (Poyner et al., 1998).
The ability of αCGRP to displace I-αCGRP was investigated on mutant receptors that were either found to have a significantly different mean pEC50 and/or agonist-mediated internalization when compared to WT (see Table 6, Fig. 4).
Although the ability of NBI-31772 to displace I-labeled IGF-1 from the other IGFBPs has not been checked in this study, it has been previously shown that it can interact with all six IGFBPs with similar efficacy [ 32].
To assess the effects of the D112N, D195N and E196Q mutations on the ligand binding sites of Cp#1 and Cp 3, we examined the ability of the compounds to displace I-CXCL10 from transfectants expressing the mutant constructs, albeit with some experimental limitations.
While the D195N mutant behaved essentially as WT CXCR3 in these assays (EC50 values of 3.2 nM and 8.2 nM respectively), the ability of Cp#3 to displace I-CXCL10 from cells expressing the E196Q mutant was significantly impaired, with less than 50% of the radiolabel displaced with a 3000-fold molar excess of Cp#3.
