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Dispersed bone marrow cell preparations were stained in FACS buffer for 15-30 min on ice with selected multicolor antibody cocktails (see below), washed, and resuspended with streptavidin conjugates when necessary.
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T-LGLs were dispersed in the bone marrow and they did not lose any pan-T-cell antigens in FCM.
In patients with polyclonal T-LGL lymphocytosis, T cells were dispersed in the bone marrow and the expression of pan-T-cell antigens in FCM was normal.
In two cases (patients 10 and 11) with polyclonal T-LGL lymphocytosis, CD3+CD8+CD57+/-/granzyme B+/- lymphocytes were dispersed in the bone marrow and did not form clusters or intravascular infiltrations.
We're mortified that our own remains might be disturbed, our coffins broken up and our bones dispersed.
Lavish mosaics, a rare find in Rome's catacombs, had been pulled from the walls, "the marble incrustations torn off, the altar dismantled, the bones dispersed".
The viol fragments show traces of two different kinds of decorations: brush painted areas, especially present on the scroll and historically made with carbon black or black bone particles dispersed in an organic medium, and purfling inlays, on the upper block of the neck and on the back plate, generally made by black wooden strips colored by iron-gallic dye.
"They refer to the surface of the water," Mr. Recordon said, "litter and offerings floating and dispersing — flowers, human bone and ashes, animal carcasses".
Bone fragments are dispersed centrifugally in all directions, including into the spinal canal, often resulting in catastrophic neural damage.
BMC were harvested from the tibia or femur by short centrifugation of the bone, and were dispersed in PBS by repeated pipetting.
There are no associations of elements belonging to the same individual, which suggests that, prior to or during debris-flow transport, bones were profusely dispersed.
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