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Rheology studies were then conducted to determine the effects of crosslink flow on filament width, hydrogel dispensing pressure on filament width, and dispensing time interval on spheroid diameter.
The cell viability was calculated by counting live or dead cells for each image at each dispensing pressure (n = 8).
If the beer doesn't foam at 12 PSI, see if it's OK at 14 PSI, which is often a fine dispensing pressure (unless the beer foams at this higher pressure).
Pyrosequencing [ Ronaghi et al., 1998] was performed on an automated plate-based benchtop pyrosequencing (PSQ) system (Uppsala, Sweden) at a dispensing pressure of 625 mbar with 4-msec open time and 65-sec cycle time.
When for instance dispensing on the ground floor or even first floor from kegs stored in a cool cellar, the necessary extra 0.3 bar pressure per floor level can be added to the "normal" dispensing pressure without any negative effects of over-carbonization.
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Living cells (i.e., mouse 3T3-J2 fibroblast) were dispensed with different dispensing pressures in order to evaluate the effect of dispensing process on cell viability and proliferation.
a) Dispensing software, showing a force distance curve a droplet is a height of approximate 50 nm (~3.5 fL), b) Controllable parameters, with the Z-position settings (top panel) and dispense pressure settings (bottom panel).
The high injection dispense pressure (∼60 Psi) and piezo vibration also help to remove any material from the tip during normal operation.
If the needle clogs during injections, visualize the clog by adjusting the focus knob, manually adjust the injection dispense pressure to maximum pressure (∼90 Psi) and the dispense duration to 10 sec.
Therefore, the KeyKeg has great benefits when a higher dispense pressure is needed.
The alginate-dispensing pressure was set at 82.7 kPa, while the CaCl2 dispensing rate was set at 16 ml/min.
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