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Extrusion bioprinting has exciting prospects in fabricating organized tissue constructs by simultaneous dispensing of cells and matrix materials to repair or replace damaged or diseased tissues and organs.
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Fig. 3 Schematic views of the cell proliferation test procedure: a dispensing of cell droplets into the well, b addition of the CCK-8 solution into the well and measurement of light absorbance after incubation, and c replacement with fresh cell media.
Administering or dispensing of narcotic drugs.
After dispensing the cells, 10 µL of CCK-8 solution was added to each well and the plates were incubated for 2 h at 37 °C in an atmosphere containing 10% CO2 (Fig. 3b).
Fig. 2 Schematic views of the cell viability test procedure: a injection of the collagen solution into the well, b thermal gelation of the collagen solution, and c dispensing of a cell-containing droplet.
This paper presents results for dispensing living cells using a pneumatic dispensing system to verify the feasibility of using this system to fabricate biomaterials.
This paper presents results for dispensing living cells using a pneumatic dispensing system to verify the feasibility of using it as a biocompatible fabrication tool.
This established the proper height for dispensing suspended cells.
Samples were measured repeatedly for continuous monitoring of the same sample at each time point (4, 24, 48, and 72 h) after dispensing the cells.
To test the biocompatibility of our dispensing system, cell viability and proliferation were analyzed qualitatively using live-dead and CCK-8 assays.
Living cells (i.e., mouse 3T3-J2 fibroblast) were dispensed with different dispensing pressures in order to evaluate the effect of dispensing process on cell viability and proliferation.
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